In modern biomanufacturing, culture media quality directly dictates cell viability and overall process yields. Traditional batch sterilization in fermenters or preparation tanks often causes thermal degradation and nutrient loss due to prolonged heating exposure. Continuous media sterilization before fermentation eliminates these issues by heating the liquid rapidly to precise temperatures, protecting delicate nutrients, and maximizing bioreactor availability for actual production cycles.
ACTINI continuous media sterilizers use High-Temperature Short-Time (HTST) thermal treatment to deliver complete sterility while preserving sensitive media components—giving your microorganisms the optimal growth environment every time.
Our continuous tubular systems handle variable-viscosity culture media from 1 to 1,000 cP without clogging or fouling.
Carbon & sugar sources: Glucose, sucrose, and complex carbohydrate feedstocks (prevents caramelization).
Complex nitrogen & nutrient broths: Yeast extracts, peptones, proteins, and agar preparations.
Salt & mineral solutions: Balanced growth media and buffered salt preparations.
Active & biological ingredients: Delicate peptides, growth factors, and biological additives.
Precision temperature profiles (194°F to 302°F / 90°C to 150°C) with short, exact holding times ensure complete inactivation of contaminant microbes while preserving essential vitamins, amino acids, and heat-sensitive carbon sources.
Continuous thermal processing utilizes incoming cold raw media to cool down outgoing hot sterilized media. This heat recovery section cuts utility energy consumption by up to 95%, drastically reducing steam and chilled water costs compared to traditional batch cooking.
Sterilizing media continuously on-the-fly directly into the receiving vessel eliminates in-tank heating and cooling cycle times, increasing your bioreactor utilization and annual production throughput.
Fully automated PLC management ensures identical, repeatable sterilization recipes, eliminating batch-to-batch variability with automated CIP (Clean-in-Place) and SIP (Steam-in-Place) sequences.
Scalable flow capacities from 2.3 GPM (528 gal/h / 2,000 L/h) to 46.5 GPM (10,560 gal/h / 40,000 L/h)
A compact, plug-and-play skid ideal for standard liquid media prep. Engineered for rapid delivery and straightforward operation.
Product-Contact Section: 316L stainless steel hot sections.
Holding Section: FDA-approved flexible silicone hose.
Surface Finish: Internal finish Ra ≤ 40 µin (1.0 µm) [Options down to Ra ≤ 20 µin / 0.5 µm].
Electrical Rating: NEMA 12 / IP54 electrical enclosure with standard automation.
Integration: Pre-configured for direct bioreactor line coupling.
Fully customized continuous sterilization skids designed for complex biopharma processes and strict plant standards.
High-Grade Metallurgy: Options for 316L, AL6XN, or Hastelloy C22.
Rigid Piping: Stainless steel rigid piping for holding sections.
Leak Protection: Optional sterile-side overpressure in heat recovery section to prevent cross-contamination.
US Compliance: cGMP, ASME BPE, and 21 CFR Part 11 compliant with complete traceability matrix documentation.
Safety & Control: NEMA 4X enclosure options with integrated SIL Safety PLC capability.
Feature / Specification |
STERIL’ONE RANGE |
STERIL’FLEX RANGE |
|---|---|---|
| Primary application | Standard industrial media sterilization | Customized advanced bioprocess integration |
| Flow capacities | 2.3 to 46.5 GPM (528 to 10,560 gal/h) | 2.3 to 46.5 GPM (528 to 10,560 gal/h) |
| Design & engineering | Standardized 3D, hydraulic & electrical | Custom-designed to plant requirements |
| Automation & PLC | Standard Automation | Configured / Optional SIL Safety PLC |
| Product contact steel | 316L Stainless Steel | 316L / AL6XN / Hastelloy C22 |
| Heat recovery type | 316L simple plate sanitary type | Custom plates / Compabloc titanium |
| Holding section connection | FDA-approved flexible silicone hose | Rigid stainless steel piping |
| Internal surface finish | Ra ≤ 40 µin (1.0 µm) standard | Ra ≤ 32 µin (0.8 µm) / Ra ≤ 20 µin (0.5 µm) |
| Sterile-side overpressure | No | Optional (Yes) |
“Made in France” Expertise: All our equipment is designed and manufactured in our French workshops, guaranteeing full traceability and responsive support.
Proven Track Record: With over 130 projects completed worldwide every year, we have mastered the constraints of the most demanding environments (BSL3/BSL4 laboratories, biopharma sites).
Customized Support: From thermal kinetic analysis to fleet maintenance, our engineers optimize your operating costs (OPEX) and process safety.
De-risk your scale-up. Test continuous HTST sterilization on your specific media formulations before full plant integration. ACTINI pilot units are available for trial runs at our test facility or directly at your site in the US.
Our engineers can help you calculate your peak flow rates and select the ideal sterilization cycle for your specific biological agents.
